Effects of Square Stepping Exercise on cognitive, physical, psychological, and group functioning in sedentary older adults: A center-based hybrid trial.

BACKGROUND: The Square Stepping Exercise (SSE) is an exercise training program that integrates physical exercise and cognitive elements and can be conducted in a group setting. The potential of SSE in delaying cognitive decline in older adults is promising. However, the coronavirus pandemic has made it more difficult for older adults worldwide to exercise together in person. To address this issue, this study conducted a wholistic evaluation of the effects of a center-based hybrid SSE trial on cognitive, physical, psychological, and group functioning in sedentary older adults. METHODS: A total of 93 older adults (19 men, 74 women) participated in the study. Fifty-eight participants (9 men, 49 women) completed center-based hybrid SSE sessions over 12 weeks under coronavirus pandemic circumstances, whereas other 35 participants in the control group maintained their current level of daily activities. Cognitive functions focused on executive functions assessed by the Stroop Color-Word Test (inhibition) and the Trail Marking Test (TMT) (set-shifting). Psychological and group functioning were assessed by the Subjective Vitality Scale and the Physical Activity Group Environment Questionnaire. Physical function was evaluated by measuring gait speeds. A repeated ANOVA was conducted on the measured variables separately for the intervention and control groups to focus on the change of participant's performance over data collection points. RESULTS: Outcomes of the Stroop Color-Word Test and the TMT revealed that the hybrid SSE was highly effective in improving executive function. Stroop performance (correct trials) was significantly improved in the incongruent condition, as well as both TMT-A and -B over the intervention period in the intervention group. The hybrid SSE was also beneficial to improve physical (gait speed at usual pace and at the maximum pace) as well as psychological functioning (subjective vitality). Furthermore, SSE participants reported increased engagement with the SSE task, social communication, and increased bonding and closeness with their group members through the hybrid SSE. CONCLUSIONS: In this study, hybrid SSE was found to be effective in enhancing cognitive, physical, psychological, and group functioning in sedentary older adults. The findings of this study are crucial to provide older adults with a safer and efficient option to exercise.

Timing of Caffeine Ingestion Does Not Improve Three-Point Shooting Accuracy in College Basketball Players.

This study investigated the effects of the timing of caffeine (3 mg/kg body mass) ingestion on three-point shooting accuracy and other performance parameters during a basketball exercise simulation test (BEST). Eighteen college basketball players (mean ± SD: age = 24.4 ± 1.5 years, height = 181.7 ± 9.5 cm, body mass = 80.9 ± 13.2 kg) underwent one familiarization trial and three main conditions in a randomized order: (a) placebo (maltodextrin) and placebo, (b) caffeine and placebo, and (c) placebo and caffeine. Participants ingested either the placebo or caffeine pill 75 and 15 min before performing four quarters of the BEST and a three-point shooting protocol. During each quarter, participants completed 16 rounds of the BEST and ten three-point shots. Vertical jump height, 6 m sprint timing, BEST completion timing, three-point shooting accuracy, heart rate, rate of perceived exertion, blood glucose, blood lactate, and psychological measures pertaining to performance were measured. The BEST completion timing differed among conditions (placebo and placebo = 26.4 ± 2.0 s, caffeine and placebo = 25.8 ± 2.0 s, placebo and caffeine = 25.9 ± 2.1 s; p = .031) but not three-point shooting accuracy (placebo and placebo = 12.33 ± 4.10; caffeine and placebo = 12.61 ± 2.81; placebo and caffeine = 11.67 ± 3.77; p = .648), vertical jump height, or sprint times. Manipulating ingestion timing of caffeine did not improve three-point shooting accuracy, vertical jump height, or 6 m sprint timings, but caffeine can improve performance times during simulated basketball exercise irrespective of ingestion timing.

Developing Nutrition Knowledge and Attitude Measures for Athletes With the Knowledge-Attitude-Behavior Model.

Purpose: Nutrition education programmes are often created to improve dietary intake in athletes. Unfortunately, the inconsistent link between nutrition knowledge and dietary behavior suggests that nutrition knowledge may not be a key influencer of dietary behavior. The Knowledge-Attitude-Behavior (KAB) model has been used in nutrition education although studies that evaluated this model were largely compromised by poorly constructed instruments and methodological discrepancies. Method: To improve understanding of the model, a Nutrition Knowledge Questionnaire (NKQ) and Athlete's Perspective on Nutrition (APN) instrument was developed using the theory of planned behavior as its theoretical framework. Validity and reliability were examined for 193 Sports Science students and 11 elite netballers. Content and face validity of the 48-item NKQ was confirmed by expert sport dietitians. Discriminant validity of the NKQ and factorial validity of the APN was established. Both instruments demonstrated acceptable internal consistency and good test-retest reliability. The final instruments were administered to elite athletes in a pilot study to understand the KAB model. Results: Correlation between nutrition knowledge and dietary behavior was significant (r = .811, p = .01). Nutrition knowledge was a positive predictor of netballers' dietary behavior (ß = .8, t(2) = 3.899, p = .005) but attitude was not (ß = -.097, t(2) = -.471, p = .65). Conclusion: Two psychometrically sound instruments developed in this study can be used to measure nutrition knowledge and attitude in an Asian group of athletes. The pilot study with athletes showed that nutrition knowledge predicted dietary behavior.

Weekday breakfast habits and mood at the start of the school morning.

BACKGROUND: Good nutrition, regular physical activity, and adequate sleep are important for promoting health. However, it is not well characterized how these lifestyle behaviours are associated with mood at the start of school days. AIM: To identify the weekday health-related behaviours (breakfast intake, physical activity, and sleep) of Singaporean adolescents and explore their associations with mood at the beginning of the school morning. METHODS: 365 adolescents (52.3% boys, 47.7% girls) aged 14-19 years (M = 16.9, SD = 1.3 years) completed a survey set on breakfast habits, physical activity participation, sleep duration, and mood. RESULTS: More than one-third (38.6%) of participants never (15.0%) or irregularly (23.6%) consumed breakfast on weekdays. Adolescents consuming breakfast irregularly reported significantly higher negative mood scores than regular breakfast consumers (p = .002; d = .404). Almost two-thirds (64.1%) of adolescents did <60 min of moderate-to-vigorous physical activity (MVPA) on weekdays and reported lower positive morning mood scores than those who ≥60 min of MVPA (p = .014; d = .284). Although most (88.3%) adolescents slept <8 h each night, sleep duration was not related to either positive mood or negative mood. CONCLUSION: The present study suggests that eating breakfast on a regular basis and completing for ≥60 min of weekday MVPA are useful behaviours to start school days in a better mood. Parents and health educators should recognise the importance of establishing these habits and support students by instigating practical approaches so they can achieve a regular breakfast intake and physical activity engagement.

Correction: Tan et al. A Systematic Review of the Effects of Caffeine on Basketball Performance Outcomes. Biology 2022, 11, 17.

The authors wish to make a correction to the published version of the paper [...].

Comparison of In Vivo Intradiscal Pressure between Sitting and Standing in Human Lumbar Spine: A Systematic Review and Meta-Analysis.

BACKGROUND: Non-specific low back pain (LBP) is highly prevalent today. Disc degeneration could be one of the causes of non-specific LBP, and increased intradiscal pressure (IDP) can potentially induce disc degeneration. The differences in vivo IDP in sitting and standing postures have been studied, but inconsistent results have been reported. The primary objective of this systematic review is to compare the differences in vivo IDP between sitting and standing postures. The secondary objective of this review is to compare effect size estimates between (1) dated and more recent studies and (2) healthy and degenerated intervertebral discs. METHODS: An exhaustive search of six electronic databases for studies published before November 2021 was conducted. Articles measuring in vivo IDP in sitting and standing postures were included. Two independent researchers conducted the screening and data extraction. RESULTS: Ten studies that met the inclusion criteria were included in the systematic review, and seven studies with nine independent groups were included in meta-analyses. The sitting posture induces a significantly higher IDP on the lumbar spine (SMD: 0.87; 95% CI = [0.33, 1.41]) than the standing posture. In studies published after 1990 and subjects with degenerated discs, there are no differences in vivo IDP between both postures. CONCLUSIONS: Sitting causes higher loads on the lumbar spine than standing in the normal discs, but recent studies do not support this conclusion. Furthermore, the degenerated discs showed no difference in IDP in both postures.

Exploring the Dimensions of Movement-Specific Reinvestment From Personal Characteristics Perspectives.

The purposes of the present study were three-fold: to examine (a) if the movement-specific reinvestment responses should be represented as two dimensional constructs, (b) whether dichotomization of the movement-specific reinvestment responses are appropriate, and (c) how the two dimensions are associated with relevant psychological concepts. To conduct a comparative examination of the MSRS structure in two Asian samples, participants were 236 Japanese university students (136 men, 100 women; M age = 18.0, SD = 1.6) and 328 Singaporeans (167 men, 161 women; M age = 21.8, SD = 1.8). After examining the factor structure of the movement-specific reinvestment responses for the first purpose, latent class factor analysis was conducted for both samples for the second purpose. For the third purpose, correlation analysis and mediation analysis were conducted for a part of the Singaporean sample. Through a series of latent class factor analysis, four and three classes were identified for the Japanese and Singaporean samples, respectively. For both samples, the patterns of the item-average scores for the two movement-specific reinvestment dimensions were parallel among the classes. Conscious Motor Processing was positively associated with mental toughness, intrinsic regulation, integrated regulation, mastery-approach and task goal orientations, and dispositional flow, whereas Movement Self-Consciousness was positively related with stress and mastery-avoidance goal orientation. The findings of the study supported (a) the two-dimensional representation of the movement-specific reinvestment responses, but did not fully support (b) the practice of dichotomization of the movement-specific reinvestment responses, and indicated that (c) at the trait level, Conscious Motor Processing and Movement Self-Consciousness were associated with positive and negative psychological constructs, respectively.

Acute effects of Square Stepping Exercise on cognitive and social functions in sedentary young adults: a home-based online trial.

BACKGROUND: The Square Stepping Exercise (SSE) is an exercise training program incorporating cognitive and physical exercise components, which was originally developed for older adults to reduce falling risks. SSE's potential in delaying cognitive decline in older adults seems to be promising. However, there is scarce research on the SSE program with young adults. Furthermore, the outbreak of coronavirus disease has imposed people to change their lifestyle and behaviors, including exercise behaviors. Hence, the purpose of this study was to examine the acute effects of a home-based online SSE trial on cognitive and social functions in sedentary young adults. METHODS: A total of 18 young adults (6 males, 12 females) participated in the present study. They completed two exercise conditions (SSE and active control exercise), consisting of 3 sessions per week, over 2 weeks. A 2 times (pre vs. post) × 2 conditions (SSE vs. active control) repeated-measures ANCOVA was conducted on the score of the Modified Card Sorting Task with age and education year as covariates. A one-way repeated-measures MANOVA was performed on the subscale scores of the Physical Activity Group Environment Questionnaire to examine the effects of the exercise conditions (SSE vs. active control) on group cohesion. RESULTS: SSE was found effective to improve executive function such as abstract reasoning, mental flexibility, and problem-solving skills. Furthermore, participants' perceptions of social interaction with their group, and closeness and bonding existing in their group were significantly higher in the SSE condition than the active control condition. CONCLUSIONS: In the present study, SSE was conducted online and found to be effective to enhance executive function and group cohesion in sedentary young adults. These novel approach and findings are the strengths of the present study. People aged 60 years and over are more vulnerable to the coronavirus and at higher risk of developing serious illness. Given the coronavirus pandemic circumstances, it is worthwhile to explore the possibility of the online SSE approach to older adults in future research.

Breakfast and Exercise Improve Academic and Cognitive Performance in Adolescents.

This study examined the combined effects of breakfast and exercise on short-term academic and cognitive performance in adolescents. Eighty-two adolescents (64 female), aged 14-19 years, were randomized to four groups over a 4-hour morning: (i) a group who fasted and were sedentary (F-S); (ii) a group who ate breakfast but were sedentary (B-S); (iii) a group who fasted but completed a 30-min exercise bout (F-E); and (iv) a group who ate breakfast and completed a 30-min exercise bout (B-E). Individuals completed academic and cognitive tests over the morning. Adolescents in B-E significantly improved their mathematics score (B-E: 15.2% improvement on correct answers, vs. F-S: 6.7% improvement on correct answers; p = 0.014) and computation time for correct answers (B-E: 16.7% improvement, vs. F-S: 7.4% improvement; p = 0.004) over the morning compared with the F-S group. The B-E group had faster reaction times for congruent, incongruent and control trials of the Stroop Color-Word Task compared with F-S mid-morning (all p < 0.05). Morning breakfast and exercise combine to improve short-term mathematical task performance and speed in adolescents.

A Systematic Review of the Effects of Caffeine on Basketball Performance Outcomes.

Caffeine is an ergogenic aid in many sports, including basketball. This systematic review examines the effects of caffeine on basketball-related skill tasks along with physical aspects of performance deemed important for the game. A systematic search was conducted across three databases (PubMed, SPORTDiscus and Web of Science) to identify randomized-controlled trials which examined the effect of caffeine on basketball performance outcomes including: free-throw, 3-point shooting accuracy, dribbling speed, vertical jump height, and linear and repeated sprints. Forty-six articles were identified of which 10 met the inclusion criteria. Improvements in vertical jump were identified in four of five studies, agility in two of four studies, and in linear and repeated sprints in two of three studies. No deterioration in basketball skills performance was observed in any studies. It is suggested that caffeine is useful for basketball players to improve the physical aspects of their game-play performance but there is little evidence of any change in skill-based performance at present. Further research should clarify the effects of caffeine on basketball performance in women and the role of individual genetic variation on caffeine metabolism. Basketball players and coaches should be aware of the properties of caffeine before ingesting it as an ergogenic supplement.

A multiple mediation analysis of the association between asynchronous use of music and running performance.

The purpose of this study was to examine if the effects of asynchronous use of music on running performance are directly and/or indirectly carried through psychological, psychophysical, and psychophysiological benefits. Following a standardized music selection procedure, 20 healthy undergraduate students (10 males, 10 females) completed a 30-min treadmill run at perceived moderate intensity while listening to music (135-140 beats per minute) or under a no-music condition in a randomized order. Participants' affective valence, arousal, perceived exertion, heart rate during the run, and running distance were measured in both trials. A mediation analysis was conducted for 40 data collected from the 20 participants under two conditions (no music; music) with a parallel mediation model in which affective valence, arousal, heart rate, and perceived exertion were specified as mediators. A significant indirect effect of affective valence was observed for running distance. The present finding supports the notion that the relationship between asynchronous use of music and running distance is mediated by psychological benefits.

Evolving the validity of a mental toughness measure: Refined versions of the Mental Toughness Questionnaire-48.

The Mental Toughness Questionnaire-48 (MTQ48) is a 48-item self-report instrument to measure one's level of mental toughness. Despite its wide popularity in psychological studies, the questionnaire has been criticized due to its factorial validity. The present study aimed to re-assess the factorial validity of the instrument and propose alternative models to provide researchers with theoretically and practically useful instruments to measure mental toughness. Two studies were conducted using large samples of university students (Study 1: n = 2186; Study 2: n = 3209). In Study 1, none of one-, four- and six-factor models with 48 items satisfactorily fit the data set. Instead, two refined 18- and 6-item versions of the questionnaire, covering six aspects of mental toughness, were proposed: the Short MTQ and Very Short MTQ. Both measures demonstrated excellent fit to the data. These results were replicated with a larger independent sample in Study 2. With the Short MTQ, it is possible to represent mental toughness as a multidimensional construct consisting of a global mental toughness factor and six specific factors. The Very Short MTQ is a practical tool for occasions where constraints prevent use of the Short MTQ. The refined questionnaires are promising options to measure and understand individuals' mental toughness with the MTQ.

Effect of Post-Exercise Massage on Passive Muscle Stiffness Measured Using Myotonometry - A Double-Blind Study.

It is commonly believed that massage can reduce muscle stiffness and is desirable for recovery from exercise. However, the effect massage on muscle stiffness following eccentric exercises is currently unknown. This study aimed to examine the effect of post-exercise massage on passive muscle stiffness over a five-day period. A randomised cross-over study design was adopted. After 40 minutes of downhill running, 18 male recreational runners had one leg received a 16-minute massage and the contralateral leg received a 16-minute sham ultrasound treatment. Passive stiffness for four leg muscles (rectus femoris, biceps femoris, tibialis anterior, and gastrocnemius) was assessed using myotonometry at baseline, immediately post-run, post-treatment, 24, 48, 72, and 96 hours post-run. A 2 (treatment) × 7 (time) mixed ANOVA was conducted with a robust procedure on the myotonometry data of each leg muscle to examine the effect of treatment on stiffness. Passive stiffness for all muscles changed over time but no treatment effect was found. Stiffness increased at 24 hours post-run and remained elevated from baseline levels for up to 96 hours across all four muscles. Significant treatment × time interaction was only found in the tibialis anterior but no post-hoc differences were identified. Passive stiffness of major leg muscles increased after a bout of unaccustomed eccentric exercise and remained elevated for up to four days post-exercise. Compared with the placebo treatment, post-exercise massage had no beneficial effect in alleviating altered muscle stiffness in major leg muscles.

A school-based intervention program in promoting leisure-time physical activity: trial protocol.

BACKGROUND: Regular participation in moderate-to-vigorous physical activity (MVPA) is important to manage obesity. Physical education (PE) is considered to play an important role in promoting lifelong participation in physical activity (PA) because it provides an existing network where cost-effective interventions can be implemented to produce sustainable change in health behavior. However, the association between compulsory school PA (e.g., PE lessons) and body composition levels has received mixed support in the literature. Therefore, the present study aimed to investigate whether a school-based intervention targeting salient PA benefits and barriers grounded on the theory of planned behavior would promote young people's participation in MVPA during leisure time and reduce body mass index (BMI) of overweight students. METHODS/DESIGN: A total of 171 students from 3 secondary schools in Singapore underwent the control condition followed by the intervention condition. Both the conditions consisted of PE lessons twice per week over 4 weeks. In the control condition, PE teachers encouraged students to participate in PA during leisure time without providing persuasive message. While in the intervention condition, PE teachers delivered persuasive messages that targeted the salient benefits and barriers associated with PA to the students at the last 5 to 10 min of each PE lesson. PA levels over a week were measured objectively with wrist-mounted GENEActiv Original accelerometers and subjectively with self-reporting questionnaires three times (Baseline, Post 1, and Post 2) in each condition. Student's self-reported PA level was measured using the Leisure-Time Physical Activity Participation Questionnaire and the International Physical Activity Questionnaire, and their attitudes, intentions, subjective norms and perceived behavior control towards leisure-time PA were measured with a questionnaire based on the theory of planned behavior. Furthermore, students' intention, determination and willingness to engage in leisure-time PA were compared with the other activity (e.g., doing homework, shopping). DISCUSSION: This study will provide the evidence on the effectiveness of a cost-effective school-based intervention on reducing BMI of overweight students through promoting sustained participation in leisure-time PA. It will also address methodological issues on the gaps between objective and subjective measures of PA. TRIAL REGISTRATION: This trial is registered with the ISRCTN registry (ISRCTN73786157, 26/10/2017, retrospectively registered).

Prediction of fruit and vegetable intake: The importance of contextualizing motivation.

OBJECTIVES: Motivation is identified as a key antecedent of self-regulated behaviour, such as eating fruit and vegetables. However, inaccurate measurement of this construct may lead to poor prediction of behaviour and inflate the impact of post-motivational factors, such as planning, in models of health behaviour. This study explored the properties of a newly identified measure of motivation, termed behavioural resolve (Rhodes & Horne, 2013, Psychol. Sport Exerc., 14, 455-460), in relation to intention, planning, and fruit and vegetable intake (FVI). DESIGN: Prospective self-report survey. METHOD: University students living in the United Kingdom completed two online surveys. The first assessed demographic and predictor variables (intention, behavioural resolve, action planning, and coping planning). The second, completed approximately 2 weeks later, measured average daily FVI and perceived experience of obstacles to FVI. At Time 1, there were 195 respondents, with 139 providing follow-up data. RESULTS: All predictor variables were significantly correlated with FVI. Two independent multiple hierarchical regression analyses revealed that both intention and behavioural resolve were significant predictors of FVI, but behavioural resolve explained greater FVI variance (40.1%) than intention (36.4%). Furthermore, action planning showed incremental predictive utility over intention, but not behavioural resolve, in predicting FVI. CONCLUSIONS: The results indicated that motivation is an important determinant of FVI for students, with behavioural resolve demonstrating advantages over intention as a measure of this domain and a predictor of FVI behaviour.

Evaluation of disaster preparedness training and disaster drill for nursing students.

BACKGROUND: Preparedness and preventive measures are needed to reduce the impact of disasters. Disaster preparedness training for nurses has a long history. However, the effectiveness of disaster preparedness training for nursing students has been limited, to some extent, since they have been based on self-evaluation. OBJECTIVE: The study attempts to evaluate the effectiveness of a disaster preparedness training program followed by a disaster drill designed for nursing students. PARTICIPANTS: Participants were undergraduate students from Universitas Gadjah Mada and diploma students from four randomly chosen nursing colleges located in Yogyakarta, Indonesia. 309 students participated in the training program whereas 225 students participated in the disaster drill. METHODS: The present study conducted in-class training followed by a disaster drill and evaluated using 3 components: pre-test and post-test evaluation of knowledge (score range: 0-20), observation of skills during disaster drill (5-point Likert scale), and a structured one-to-one interview of participants' responses to the training and drill process. RESULTS: Pre-test and post-test evaluation scores showed significant improvement (P<0.01, paired t-test) for both university and diploma students. Almost all observation items during the disaster drill were above 4.0 (on 5-point Likert scale). Interview results showed that most participants responded positively. CONCLUSIONS: The present study completely evaluated the effectiveness of a disaster preparedness training and disaster drill: The training and drill improved the knowledge and ability of disaster preparedness for both undergraduate and diploma students.

Sonic hedgehog and androgen signaling in tumor and stromal compartments drives epithelial-mesenchymal transition in prostate cancer.

OBJECTIVE: Sonic hedgehog (Shh) signaling, androgens and epithelial-mesenchymal transition (EMT) are related to prostate cancer (PCa) progression. The aim of this study was to investigate how Shh and androgen [dihydrotestosterone (DHT)] signaling act in prostate epithelial and stromal compartments and whether this signaling pathway drives EMT and promotes PCa progression. MATERIAL AND METHODS: LNCaP, normal prostate fibroblast (NPF) and cancer-associated prostate fibroblast (CPF) cells were studied with DHT and/or the Shh signaling inhibitor cyclopamine. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate the expressions of a potential Shh target gene, osteonectin (ON) and EMT-associated markers (E-cadherin, N-cadherin and vimentin). Immunohistochemical studies using PCa prostatectomy samples were performed to assess the expression levels of ON, Gli-1, androgen receptor, Shh, E-cadherin, N-cadherin and vimentin. RESULTS: While DHT enhanced cell proliferation in CPF more than LNCaP or NPF, cyclopamine inhibited cell proliferation enhanced by DHT in CPF. Real-time RT-PCR showed whereas both Shh and DHT induced N-cadherin and vimentin, DHT also induced the expression of osteonectin in LNCaP and cyclopamine blocked these expressions in osteonectin, N-cadherin and vimentin (p = 0.0084, 0.0002 and 0.0373, respectively). Immunohistochemistry showed that high expression of stromal, but, not epithelial, ON was significantly correlated with serum prostate-specific antigen (PSA) (p = 0.031), and high expression of Gli-1 and low expression of stromal ON with PSA recurrence (p = 0.0114 and p = 0.0005, respectively). CONCLUSIONS: Shh and androgen signaling in prostate tumor and stromal compartments drives EMT, and thus may play some role in PCa progression. Cyclopamine may be one therapeutic strategy for PCa.

Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice.

More than 170 million people worldwide are chronic HCV (Hepatitis C virus) carriers, and about 30% of them will develop progressive liver disease, such as cirrhosis and hepatocellular carcinoma. A combination of pegylated interferon-α with ribavirin, the standard treatment for HCV infection, has been effective in fewer than 50% of patients infected with HCV genotype 1. A strong T cell response against the nonstructural protein 3 (NS3) is important for recovery from acute HCV infection, and an early multi-specific CD4+ helper and CD8+ cytotoxic T cell response is critical for HCV clearance. In the present study, we successfully constructed a genetically modified Bifidobacterium longum (B. longum) displaying recombinant HCV-NS3 peptides containing some CD4 and CD8 epitopes located in the HCV-NS3 region as an oral vaccine against chronic HCV infection. The oral administration of this vaccine could induce NS3-specific immune responses in mice through intestinal mucosal immunity. Our findings suggest that this novel oral vaccine has great potential as a novel oral vaccine against chronic HCV infection.

Possible role of sonic hedgehog and epithelial-mesenchymal transition in renal cell cancer progression.

PURPOSE: Sonic hedgehog (Shh) signaling and epithelial-mesenchymal transition (EMT) are both known to relate to cancer progression. The purpose of this study was to investigate the role of Shh signaling and EMT in renal cell carcinoma (RCC). MATERIALS AND METHODS: Cell proliferation was assayed in RCC cell lines in the presence or absence of a Shh signaling stimulator, recombinant Shh (r-Shh) protein, or a Shh signaling inhibitor, cyclopamine. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to study the expression of EMT markers (E-cadherin, N-cadherin, and vimentin) and osteonectin. The expression of Ki-67, Gli-1, osteonectin, and EMT markers in nephrectomy specimens from RCC patients was also measured by immunohistochemical (IHC) staining. RESULTS: RCC cells showed enhanced cell proliferation by r-Shh protein, whereas cell proliferation was suppressed by the addition of cyclopamine in RenCa cells. Real-time RT-PCR showed that r-Shh suppressed the expression of E-cadherin and that this suppression was partly blocked by cyclopamine alone in RenCa cells. In the IHC results, osteonectin significantly correlated with vein sinus invasion (p=0.0218), and the expression of vimentin significantly correlated with lymphatic invasion (p=0.0392). CONCLUSIONS: Shh signaling and EMT play roles in RCC progression, and the Shh signaling inhibitor cyclopamine might be a possible molecular targeted therapeutic strategy for RCC.

Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention against head and neck cancer-containing HPV16 cell lines.

CONCLUSION: Our results indicate that siRNA E6 and/or E7 may have potential as a gene-specific therapy for human papillomavirus (HPV) type 16 (HPV16)-related squamous cell carcinoma of the head and neck (HNSCC). OBJECTIVES: To evaluate the effectiveness of siRNA targeting E6 and/or E7 on the in vitro and in vivo growth suppression of HPV16-related HNSCC. METHODS: HPV16-related HNSCC (UM-SCC47) cell lines were used for the present study. Expression of HPV viral oncogenes E6 and/or E7 and their cellular targets, p53 and pRb, was evaluated by real-time PCR, Western blotting, and immunofluorescence staining. To study the effect of siRNA on tumor growth in vivo, we developed animal models. Representative tumors harvested from each group were processed for apoptosis analyses (TUNEL assay) and immunofluorescence staining for p53 and pRb. RESULTS: E6 and E7 oncogenes of HPV16 were down-regulated by E6 and/or E7 targeting siRNAs, respectively. The expression of p53 and pRb proteins in both the E6 siRNA group and E7 siRNA group was up-regulated compared with those of control groups. The cellular proliferation and apoptosis indexes of E6 and/or E7 siRNA groups were higher than those of controls. In vivo studies showed significant inhibitory effect of E6 and/or E7 siRNA compared with those of control groups, which was consistent with in vitro studies.